Micro pumping with cardiomyocyte-polymer hybrid

Lab Chip. 2007 Oct;7(10):1367-70. doi: 10.1039/b703900j. Epub 2007 Jul 20.

Abstract

This paper presents a hybrid micropump actuated by the up-down motion of a dome shaped cell-polymer membrane composite. The contractile force induced from self-beating cardiomyocytes cultured on the membrane causes shrinkage and relaxation of a microchamber, leading to a flow in a microchannel. Flow direction is controlled by the geometry of diffuser/nozzle in the microchannel. The fabrication process is noninvasive to cells, thus, cardiomyocytes can robustly maintain their activity for a long time. The fluid motion in the microchannel was monitored by tracking 2 microm polystyrene beads. A net flow rate of 0.226 nl min(-1) was obtained in our microscale device. Our device demonstrates a unique performance of a cell-microdevice hybrid lab-on-a-chip that does not require any external power source, preventing electrical or heat shock to analytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Biomimetics / instrumentation*
  • Biomimetics / methods
  • Cell Culture Techniques / instrumentation*
  • Cell Culture Techniques / methods
  • Cells, Cultured
  • Dimethylpolysiloxanes / chemistry*
  • Equipment Design
  • Equipment Failure Analysis
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods
  • Myocardial Contraction / physiology*
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / physiology*
  • Polymers / chemistry*
  • Pressure
  • Rats
  • Rats, Sprague-Dawley
  • Silicones / chemistry*
  • Stress, Mechanical

Substances

  • Dimethylpolysiloxanes
  • Polymers
  • Silicones
  • baysilon