Anti-Ro and anti-La autoantibodies induce TNF-alpha production by human salivary gland cells: an in vitro study

Reumatismo. 2007 Jul-Sep;59(3):221-6. doi: 10.4081/reumatismo.2007.221.

Abstract

In this report, we demonstrate that both TNFR1 and the TNFR2 are expressed on the salivary gland cell line A-253 cell membrane. Furthermore, cell treatment with anti-Ro and anti-La autoantibodies from Sjögren IgG determined TNF-alpha production, clarifying which could be the inducer of the extrinsic pathway of apoptosis in salivary gland cells.

MeSH terms

  • Antibodies, Antinuclear / immunology
  • Antibodies, Antinuclear / isolation & purification
  • Antibodies, Antinuclear / pharmacology*
  • Apoptosis / physiology
  • Autoantigens / immunology*
  • Carcinoma, Squamous Cell / pathology
  • Cell Line, Tumor / metabolism
  • Gene Expression Regulation / immunology*
  • Humans
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification
  • Immunoglobulin G / pharmacology*
  • In Vitro Techniques
  • Receptors, Tumor Necrosis Factor, Type I / biosynthesis*
  • Receptors, Tumor Necrosis Factor, Type I / genetics
  • Receptors, Tumor Necrosis Factor, Type II / biosynthesis*
  • Receptors, Tumor Necrosis Factor, Type II / genetics
  • Ribonucleoproteins / immunology*
  • SS-B Antigen
  • Salivary Gland Neoplasms / pathology
  • Sjogren's Syndrome / immunology
  • Submandibular Gland / cytology*
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Tumor Necrosis Factor-alpha / genetics

Substances

  • Antibodies, Antinuclear
  • Autoantigens
  • Immunoglobulin G
  • Receptors, Tumor Necrosis Factor, Type I
  • Receptors, Tumor Necrosis Factor, Type II
  • Ribonucleoproteins
  • SS-A antibodies
  • SS-A antigen
  • Tumor Necrosis Factor-alpha