Abstract
The SAX-3/roundabout (Robo) receptor has SLT-1/Slit-dependent and -independent functions in guiding cell and axon migrations. We identified enhancer of ventral-axon guidance defects of unc-40 mutants (EVA-1) as a Caenorhabditis elegans transmembrane receptor for SLT-1. EVA-1 has two predicted galactose-binding ectodomains, acts cell-autonomously for SLT-1/Slit-dependent axon migration functions of SAX-3/Robo, binds to SLT-1 and SAX-3, colocalizes with SAX-3 on cells, and provides cell specificity to the activation of SAX-3 signaling by SLT-1. Double mutants of eva-1 or slt-1 with sax-3 mutations suggest that SAX-3 can (when slt-1 or eva-1 function is reduced) inhibit a parallel-acting guidance mechanism, which involves UNC-40/deleted in colorectal cancer.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Genetically Modified
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Axons / physiology*
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / chemistry*
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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Cell Movement
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Cloning, Molecular
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Humans
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Molecular Sequence Data
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Mutation
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Nerve Tissue Proteins / metabolism*
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Nervous System / growth & development
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Nervous System / metabolism
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Neurons / physiology
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Protein Structure, Tertiary
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Receptors, Immunologic / metabolism*
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Recombinant Fusion Proteins / metabolism
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Roundabout Proteins
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Signal Transduction
Substances
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Caenorhabditis elegans Proteins
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Carrier Proteins
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EVA-1 protein, C elegans
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Nerve Tissue Proteins
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Receptors, Immunologic
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Recombinant Fusion Proteins
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slt-1 protein, C elegans
Associated data
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GENBANK/AF358257
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GENBANK/AF358258
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RefSeq/NP_493590