Decoding protein modifications using top-down mass spectrometry

Nat Methods. 2007 Oct;4(10):817-21. doi: 10.1038/nmeth1097.


Top-down mass spectrometry is an emerging technology which strives to preserve the post-translationally modified forms of proteins present in vivo by measuring them intact, rather than measuring peptides produced from them by proteolysis. The top-down technology is beginning to capture the interest of biologists and mass spectrometrists alike, with a main goal of deciphering interaction networks operative in cellular pathways. Here we outline recent approaches and applications of top-down mass spectrometry as well as an outlook for its future.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Chromatin / chemistry
  • Mass Spectrometry / methods*
  • Membrane Proteins / chemistry
  • Protein Isoforms / chemistry
  • Protein Processing, Post-Translational
  • Proteomics / methods*
  • Tandem Mass Spectrometry / methods


  • Chromatin
  • Membrane Proteins
  • Protein Isoforms