Production of the endocannabinoids anandamide and 2-arachidonoylglycerol by endothelial progenitor cells

FEBS Lett. 2007 Oct 16;581(25):4927-31. doi: 10.1016/j.febslet.2007.09.032. Epub 2007 Sep 24.

Abstract

Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-alpha stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-alpha.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arachidonic Acids / biosynthesis*
  • Arachidonic Acids / pharmacology
  • Cannabinoid Receptor Modulators / biosynthesis*
  • Cannabinoid Receptor Modulators / pharmacology
  • Endocannabinoids*
  • Endothelial Cells / cytology
  • Endothelial Cells / drug effects
  • Endothelial Cells / metabolism
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Glycerides / biosynthesis*
  • Glycerides / pharmacology
  • Humans
  • Polyunsaturated Alkamides / pharmacology
  • Stem Cells / classification
  • Stem Cells / drug effects
  • Stem Cells / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology
  • Up-Regulation
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • Arachidonic Acids
  • Cannabinoid Receptor Modulators
  • Endocannabinoids
  • Glycerides
  • Polyunsaturated Alkamides
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • glyceryl 2-arachidonate
  • anandamide