Abstract
Recent studies have highlighted the importance of paracrine growth factors as mediators of pro-angiogenic effects by endothelial progenitor cells (EPCs), but little is known about the release of lipid-based factors like endocannabinoids by EPCs. In the current study, the release of the endocannabinoids anandamide and 2-arachidonoylglycerol by distinct human EPC sub-types was measured using HPLC/tandem mass-spectrometry. Anandamide release was highest by adult blood colony-forming EPCs at baseline and they also demonstrated increased 2-arachidonoylglycerol release with TNF-alpha stimulation. Treatment of mature endothelial cells with endocannabinoids significantly reduced the induction of the pro-inflammatory adhesion molecule CD106 (VCAM-1) by TNF-alpha.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Arachidonic Acids / biosynthesis*
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Arachidonic Acids / pharmacology
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Cannabinoid Receptor Modulators / biosynthesis*
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Cannabinoid Receptor Modulators / pharmacology
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Endocannabinoids*
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Endothelial Cells / cytology
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Endothelial Cells / drug effects
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Endothelial Cells / metabolism
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Endothelium, Vascular / cytology*
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Endothelium, Vascular / metabolism
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Glycerides / biosynthesis*
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Glycerides / pharmacology
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Humans
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Polyunsaturated Alkamides / pharmacology
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Stem Cells / classification
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Stem Cells / drug effects
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Stem Cells / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
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Up-Regulation
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Vascular Cell Adhesion Molecule-1 / metabolism
Substances
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Arachidonic Acids
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Cannabinoid Receptor Modulators
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Endocannabinoids
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Glycerides
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Polyunsaturated Alkamides
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Tumor Necrosis Factor-alpha
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Vascular Cell Adhesion Molecule-1
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glyceryl 2-arachidonate
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anandamide