Genetically delivered antibody protects against West Nile virus

Antiviral Res. 2008 Jan;77(1):6-13. doi: 10.1016/j.antiviral.2007.08.010. Epub 2007 Sep 14.


Gene-based delivery of recombinant antibody genes is a promising therapeutic strategy offering numerous advantages including sustained antibody levels, better safety profile and lower production cost. Here we describe generation of a recombinant antibody Fc-9E2 comprising a fusion protein between human Fc of IgG1 and a single-chain Fv derived from a hybridoma 9E2 secreting a mAb neutralizing West Nile virus (WNV). Fc-9E2 was shown to retain parental mAb's specificity and WNV-neutralizing capacity. Adenovirus-mediated in vivo delivery of the antibody gene resulted in sustained Fc-9E2 serum levels leading to abrogation of lethal WNV infection in an animal model.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Viral / genetics*
  • Antibodies, Viral / immunology*
  • Female
  • Gene Transfer Techniques
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Fusion Proteins / immunology
  • West Nile Fever / immunology*
  • West Nile Fever / prevention & control*
  • West Nile Fever / virology
  • West Nile virus / immunology*


  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Recombinant Fusion Proteins