Real-time measurement of pyrophosphate release kinetics

Anal Biochem. 2008 Jan 1;372(1):125-7. doi: 10.1016/j.ab.2007.08.004. Epub 2007 Aug 10.

Abstract

In order to measure the kinetics of pyrophosphate release, we have coupled pyrophosphatase to the fluorescently labeled phosphate binding protein developed by Webb. We show that the assay is capable of measuring rates of pyrophosphate release up to 100 s−1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diphosphates / metabolism*
  • Fluorescence
  • Kinetics

Substances

  • Diphosphates