Transgenesis upgrades for Drosophila melanogaster

Development. 2007 Oct;134(20):3571-84. doi: 10.1242/dev.005686.


Drosophila melanogaster is a highly attractive model system for the study of numerous biological questions pertaining to development, genetics, cell biology, neuroscience and disease. Until recently, our ability to manipulate flies genetically relied heavily on the transposon-mediated integration of DNA into fly embryos. However, in recent years significant improvements have been made to the transgenic techniques available in this organism, particularly with respect to integrating DNA at specific sites in the genome. These new approaches will greatly facilitate the structure-function analyses of Drosophila genes, will enhance the ease and speed with which flies can be manipulated, and should advance our understanding of biological processes during normal development and disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • DNA Repair
  • DNA Transposable Elements / genetics
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology
  • Gene Transfer Techniques*
  • Genetic Markers
  • Genome
  • Recombination, Genetic
  • Transgenes*


  • DNA Transposable Elements
  • Drosophila Proteins
  • Genetic Markers