Introduction: Angiotensin II (Ang II) inhibitory agents such as angiotensin-converting enyzme (ACE) inhibitors have been used as antihypertensive and anti-proteinuric drugs, attenuating the progression and inducing regression of glomerulosclerosis. The mechanisms related to the renoprotective effects of the ACE-inhibitors, although partly related to the blockade of the renin-angiotensin system (RAS), are not completely understood. Their effects on many cellular functions and intracellular components have not been directly studied. The aim of the present studies was to investigate the effect of enalapril on lysosomal activity.
Material and methods: Two groups of Wistar rats were studied. The experimental group received enalapril, 50 mg/L, in the drinking water; the control group was submitted to the same conditions except for enalapril. After two weeks of treatment, each animal was sacrificed and samples of liver and kidney were individually collected, homogenised and subjected to differential centrifugation. The supernatant was utilised for evaluation of ortophosphoric-monoester phosphohydrolase as a marker of lysosome stability and the results expressed as specific units of phosphatase activity.
Results: The mean specific activities of the lysosomal compartment isolated from kidneys of enalapril-treated rats showed a mean specific activity that was 17.6% higher than the control group; no: difference was found in the liver.
Conclusion: The present studies suggests that the beneficial effect of enalapril is not related to effects on lysosomal membrane.