Detection of pim-1 mRNA in prostate cancer diagnosis

Chin Med J (Engl). 2007 Sep 5;120(17):1491-3.


Background: Pim-1 plays an important role in the apoptosis, proliferation, differentiation of cancer cells and progression of cancer. In this study we detected the expression of pim-1 mRNA in normal prostate, benign prostatic hyperplasia (BPH), and prostate cancer (PCa) and explored its diagnostic value for PCa.

Methods: The prostate tissues were collected from 23 patients with PCa, 37 patients with BPH, and 3 healthy volunteers. Pim-1 mRNA expression levels in these samples were determined by the quantitative real-time PCR (QRT-PCR). The differences of expression were calculated based on a standard curve.

Results: The ratio of pim-1 mRNA to beta-actin in the normal prostate, BPH, and PCa were 1.05 +/- 0.04, 2.57 +/- 0.74 and 4.45 +/-0.63, respectively. The differences among PCa, BPH and NT were significant (P < 0.05, respectively).

Conclusion: Detecting pim-1 mRNA expression by QRT-PCR provides a reliable metric for the diagnosis of PCa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Prostate / metabolism
  • Prostatic Hyperplasia / metabolism
  • Prostatic Neoplasms / diagnosis*
  • Prostatic Neoplasms / metabolism
  • Proto-Oncogene Proteins c-pim-1 / genetics*
  • RNA, Messenger / analysis*
  • Sensitivity and Specificity


  • RNA, Messenger
  • PIM1 protein, human
  • Proto-Oncogene Proteins c-pim-1