Immunogenicity of a killed Leishmania vaccine with saponin adjuvant in dogs

Vaccine. 2007 Nov 1;25(44):7674-86. doi: 10.1016/j.vaccine.2007.08.009. Epub 2007 Aug 24.


Cellular and humoral immune responses of dogs to a candidate vaccine, composed of Leishmania braziliensis promastigote protein plus saponin as adjuvant, have been investigated as a pre-requisite to understanding the mechanisms of immunogenicity against canine visceral leishmaniasis (CVL). The candidate vaccine elicited strong antigenicity related to the increases of anti-Leishmania IgG isotypes, together with higher levels of lymphocytes, particularly of circulating CD8(+) T-lymphocytes and Leishmania chagasi antigen-specific CD8(+) T-lymphocytes. As indicated by the intense cell proliferation and increased nitric oxide production during in vitro stimulation by L. chagasi soluble antigens, the candidate vaccine elicited an immune activation status potentially compatible with effective control of the etiological agent of CVL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Animals
  • Antibodies, Protozoan / blood
  • B-Lymphocytes / immunology
  • Dog Diseases / prevention & control*
  • Dogs
  • Female
  • Leishmania braziliensis / immunology*
  • Leishmaniasis, Visceral / prevention & control
  • Leishmaniasis, Visceral / veterinary*
  • Lymphocyte Activation
  • Male
  • Nitric Oxide / biosynthesis
  • Protozoan Vaccines / immunology*
  • Saponins / administration & dosage*
  • T-Lymphocyte Subsets / immunology
  • Vaccines, Inactivated


  • Adjuvants, Immunologic
  • Antibodies, Protozoan
  • Protozoan Vaccines
  • Saponins
  • Vaccines, Inactivated
  • Nitric Oxide