In vitro susceptibility patterns of fungi associated with keratomycosis in horses of the northeastern United States: 68 cases (1987-2006)

J Am Vet Med Assoc. 2007 Oct 1;231(7):1086-91. doi: 10.2460/javma.231.7.1086.

Abstract

Objective: To determine in vitro susceptibility patterns of fungi associated with keratomycosis in horses in the northeastern United States and compare those patterns with results of studies from other geographic regions.

Design: Retrospective case series.

Animals: 68 horses with keratomycosis.

Procedures: Medical records of horses with a clinical diagnosis of keratomycosis, positive results of corneal fungal cultures, and susceptibility data were reviewed from the years 1987 to 2006. Fungal identification and in vitro antifungal susceptibility test results were recorded. The percentage of susceptible isolates was compared among antifungals for all isolates together and for the most common genera individually.

Results: 74 fungal isolates from 68 horses that met inclusion criteria were identified. Aspergillus, Candida, and Fusarium spp were the most frequent isolates. Grouped isolates had the highest percentage of susceptibility to nystatin (87.7%), natamycin (87.5%), and clotrimazole (80.6%). Grouped isolates had the lowest percentage of susceptibility to fluconazole (15.8%) and miconazole (27.5%). Aspergillus spp (> or = 81.0%) were most susceptible to nystatin, clotrimazole, itraconazole, and natamycin. Candida spp (100%) were most susceptible to ketaconazole, natamycin, and nystatin. Fusarium spp (100%) were only consistently susceptible to natamycin.

Conclusions and clinical relevance: On the basis of in vitro susceptibility testing, nystatin, natamycin, or clotrimazole is recommended for initial topical treatment of keratomycosis in horses from the northeastern United States. Contrary to results of studies of ocular fungal isolates of horses from other regions, Candida spp were identified more frequently and miconazole had lower in vitro efficacy in the present study.

MeSH terms

  • Animals
  • Antifungal Agents / therapeutic use*
  • Dose-Response Relationship, Drug
  • Drug Resistance, Fungal*
  • Eye Infections, Fungal / drug therapy
  • Eye Infections, Fungal / microbiology
  • Eye Infections, Fungal / veterinary*
  • Female
  • Fungi / drug effects*
  • Horse Diseases / drug therapy*
  • Horse Diseases / microbiology*
  • Horses
  • Male
  • Microbial Sensitivity Tests / veterinary
  • New England
  • Retrospective Studies
  • Treatment Outcome

Substances

  • Antifungal Agents