Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal muscle, brain and notochord

Gene Expr Patterns. 2007 Oct;7(8):827-36. doi: 10.1016/j.modgep.2007.08.001. Epub 2007 Aug 15.

Abstract

The vestigial gene has been shown to control skeletal muscle formation in Drosophila and the related Vestigial-like 2 (Vgl-2) protein plays a similar role in mice. Vgl-family proteins are thought to regulate tissue-specific gene expression by binding to members of the broadly expressed Scalloped/Tef/TEAD transcription factor family. Zebrafish have at least four Vgl genes, including two Vgl-2s, and at least three TEAD genes, including two Tead3s. We describe the cloning and expression of one member from each family in the zebrafish. A novel gene, vgl-2b, with closest homology to mouse and human vgl-2, is expressed transiently in nascent notochord and in muscle fibres as they undergo terminal differentiation during somitogenesis. Muscle cells also express a TEAD-3 homologue, a possible partner of Vgl-2b, during myoblast differentiation and early fibre assembly. Tead-3a is also expressed in rhombomeres, eye and epiphysis regions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / embryology
  • Brain / metabolism
  • Central Nervous System / chemistry
  • Central Nervous System / embryology
  • Cloning, Molecular
  • Gene Expression
  • Molecular Sequence Data
  • Muscle, Skeletal / chemistry
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / metabolism
  • Notochord / chemistry
  • Notochord / embryology
  • Notochord / metabolism
  • Phylogeny
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Zebrafish / embryology*
  • Zebrafish / genetics
  • Zebrafish Proteins / chemistry
  • Zebrafish Proteins / genetics*

Substances

  • RNA, Messenger
  • Transcription Factors
  • Zebrafish Proteins
  • tead3a protein, zebrafish
  • vgll2b protein, zebrafish