Physical and Genetic Map of the Neisseria Gonorrhoeae Strain MS11-N198 Chromosome

Mol Microbiol. 1991 Oct;5(10):2529-39. doi: 10.1111/j.1365-2958.1991.tb02099.x.

Abstract

A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Bacterial*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Neisseria gonorrhoeae / genetics*
  • Plasmids
  • Restriction Mapping

Substances

  • DNA, Bacterial