Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats

Cell Biochem Funct. Mar-Apr 2008;26(2):197-204. doi: 10.1002/cbf.1432.


Inflammation is a crucial step for the wound healing process. The effect of linoleic and oleic acids on the inflammatory response of the skin during the healing process and on the release of pro-inflammatory cytokines by rat neutrophils in vitro was investigated. A wound in the dorsal surface of adult rats was performed and fatty acids were then topically administered. Both oleic and linoleic acids increased the wound healing tissue mass. The total protein and DNA contents of the wounds were increased by the treatment with linoleic acid. The treatments with oleic and linoleic acids did not affect vascular permeability. However, the number of neutrophils in the wounded area and air pouches was increased and the thickness of the necrotic cell layer edge around the wound was decreased. A dose-dependent increase in vascular endothelial growth factor-alpha (VEGF-alpha) and interleukin-1beta (IL-1beta) by neutrophils incubated in the presence of oleic and linoleic acid was observed. Oleic acid was able to stimulate also the production of cytokine-induced neutrophil chemoattractant in inflammation 2 alpha/beta (CINC-2alpha/beta). This pro-inflammatory effect of oleic and linoleic acids may speed up the wound healing process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Chemokine CXCL2 / biosynthesis
  • Chemokine CXCL2 / drug effects
  • Dose-Response Relationship, Drug
  • Inflammation* / immunology
  • Interleukin-1beta / drug effects
  • Interleukin-1beta / immunology
  • Linoleic Acids / administration & dosage*
  • Male
  • Neutrophils / drug effects
  • Neutrophils / immunology
  • Oleic Acids / administration & dosage*
  • Rats
  • Rats, Wistar
  • Skin / drug effects
  • Skin / immunology
  • Skin / injuries
  • Vascular Endothelial Growth Factor A / drug effects
  • Vascular Endothelial Growth Factor A / immunology
  • Wound Healing / drug effects*
  • Wound Healing / immunology*


  • Chemokine CXCL2
  • Cxcl2 protein, rat
  • Interleukin-1beta
  • Linoleic Acids
  • Oleic Acids
  • Vascular Endothelial Growth Factor A