Straight GDP-tubulin protofilaments form in the presence of taxol

Curr Biol. 2007 Oct 23;17(20):1765-70. doi: 10.1016/j.cub.2007.08.063. Epub 2007 Oct 4.


Microtubules exist in dynamic equilibrium, growing and shrinking by the addition or loss of tubulin dimers from the ends of protofilaments. The hydrolysis of GTP in beta-tubulin destabilizes the microtubule lattice by increasing the curvature of protofilaments in the microtubule and putting strain on the lattice. The observation that protofilament curvature depends on GTP hydrolysis suggests that microtubule destabilizers and stabilizers work by modulating the curvature of the microtubule lattice itself. Indeed, the microtubule destabilizer MCAK has been shown to increase the curvature of protofilaments during depolymerization. Here, we show that the atomic force microscopy (AFM) of individual tubulin protofilaments provides sufficient resolution to allow the imaging of single protofilaments in their native environment. By using this assay, we confirm previous results for the effects of GTP hydrolysis and MCAK on the conformation of protofilaments. We go on to show that taxol stabilizes microtubules by straightening the GDP protofilament and slowing down the transition of protofilaments from straight to a curved configuration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Guanosine Diphosphate / chemistry*
  • Guanosine Diphosphate / metabolism*
  • Guanosine Triphosphate / metabolism
  • Hydrolysis
  • In Vitro Techniques
  • Kinesin / metabolism
  • Microscopy, Atomic Force
  • Microtubules / drug effects
  • Microtubules / metabolism
  • Microtubules / ultrastructure
  • Multiprotein Complexes
  • Paclitaxel / pharmacology*
  • Protein Conformation
  • Protein Subunits
  • Tubulin / chemistry*
  • Tubulin / drug effects
  • Tubulin / metabolism*
  • Tubulin / ultrastructure


  • Multiprotein Complexes
  • Protein Subunits
  • Tubulin
  • Guanosine Diphosphate
  • Guanosine Triphosphate
  • Kinesin
  • Paclitaxel