MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb

Cell. 2007 Oct 5;131(1):146-59. doi: 10.1016/j.cell.2007.07.021.


MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / physiology*
  • Cell Death
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Gene Expression Regulation*
  • Gene Targeting
  • Genes, Reporter
  • Humans
  • Immune System / physiology
  • Mice
  • Mice, Knockout
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Proto-Oncogene Proteins c-myb / genetics
  • Proto-Oncogene Proteins c-myb / metabolism*
  • T-Lymphocytes / physiology


  • 3' Untranslated Regions
  • MicroRNAs
  • Proto-Oncogene Proteins c-myb