Chemoprotective effect of Spirulina (Arthrospira) against cyclophosphamide-induced mutagenicity in mice

Food Chem Toxicol. 2008 Feb;46(2):567-74. doi: 10.1016/j.fct.2007.08.039. Epub 2007 Sep 5.


The aim of this study was to investigate the antimutagenic effects of Spirulina (SP) on male and female mice by the dominant lethal test using cyclophosphamide (CP) as a mutagen. Animals of both sex were given SP orally at 0, 200, 400 or 800 mg/kg body weight (b.w.) for 2 weeks prior to starting the CP treatment. CP was i.p. injected daily for 5 days at 40 mg/kg b.w. For the male-dominant lethal test, each male was caged with untreated females per week for 3 weeks. For the female-dominant lethal test the above doses and schedule treatments were used and treated females were caged for one week with untreated males (1-2). On days 13-15 after breeding was |started all the females were evaluated for incidence of pregnancy, total corpora lutea, total implants and pre- and post-implant losses. In the male-dominant lethal test, the CP induced pre- and post-implant losses in untreated females were inhibited at all SP doses. In the female-dominant lethal test only post-implantation losses were prevented at the same doses. Semen examination of a separate group of mice showed that SP improved its quality. Our results illustrate protective effects of SP in relation to CP-induced genetic damage to germ cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antimutagenic Agents / therapeutic use*
  • Cyclophosphamide / antagonists & inhibitors*
  • Cyclophosphamide / toxicity
  • Female
  • Genes, Dominant / drug effects
  • Male
  • Mice
  • Mutagens / toxicity*
  • Pregnancy / drug effects
  • Spermatozoa / drug effects*
  • Spirulina*


  • Antimutagenic Agents
  • Mutagens
  • Cyclophosphamide