Direct selection of human genomic loci by microarray hybridization

Nat Methods. 2007 Nov;4(11):903-5. doi: 10.1038/nmeth1111. Epub 2007 Oct 14.


We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • BRCA1 Protein / genetics
  • Cell Line, Tumor
  • Gene Library
  • Genome, Human / genetics*
  • Humans
  • Nucleic Acid Hybridization / methods
  • Oligodeoxyribonucleotides / genetics
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide
  • Sequence Analysis, DNA / methods


  • BRCA1 Protein
  • Oligodeoxyribonucleotides