Microarray-based genomic selection for high-throughput resequencing

Nat Methods. 2007 Nov;4(11):907-9. doi: 10.1038/nmeth1109. Epub 2007 Oct 14.


We developed a general method, microarray-based genomic selection (MGS), capable of selecting and enriching targeted sequences from complex eukaryotic genomes without the repeat blocking steps necessary for bacterial artificial chromosome (BAC)-based genomic selection. We demonstrate that large human genomic regions, on the order of hundreds of kilobases, can be enriched and resequenced with resequencing arrays. MGS, when combined with a next-generation resequencing technology, can enable large-scale resequencing in single-investigator laboratories.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Eukaryotic Cells / metabolism
  • Fragile X Mental Retardation Protein / genetics
  • Gene Library
  • Genome / genetics*
  • Genome, Human / genetics
  • Humans
  • Mutation
  • Nuclear Proteins / genetics
  • Nucleic Acid Hybridization
  • Oligodeoxyribonucleotides / genetics
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction
  • Reproducibility of Results
  • Sequence Analysis, DNA / methods*
  • Trans-Activators / genetics


  • AFF2 protein, human
  • FMR1 protein, human
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • Trans-Activators
  • Fragile X Mental Retardation Protein