The mechanism of Axl-mediated Ebola virus infection

J Infect Dis. 2007 Nov 15;196 Suppl 2:S259-63. doi: 10.1086/520594.

Abstract

We previously reported that expression of the receptor-type tyrosine kinase Axl, which regulates cell survival and activation, enhances both pseudotype and live Ebola virus (EBOV) infection. To clarify the mechanistic basis of this enhancement, we created a series of Axl mutants and identified amino acids/domains necessary for this function, by using a pseudotype virus carrying the EBOV glycoprotein (GP). Analyses of the Axl mutants showed the importance of extracellular and intracellular regions for Axl functions, including ligand binding and signal transduction, in EBOV GP-mediated infection. These data suggest that EBOV uses the physiological functions of Axl to enter cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Ebolavirus / genetics
  • Ebolavirus / pathogenicity*
  • Ebolavirus / physiology*
  • Glycoproteins / physiology
  • Humans
  • Jurkat Cells
  • Kidney
  • Mutagenesis
  • Oncogene Proteins / genetics
  • Oncogene Proteins / physiology*
  • Plasmids
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins
  • Receptor Protein-Tyrosine Kinases / genetics
  • Receptor Protein-Tyrosine Kinases / physiology*
  • Recombinant Proteins / metabolism
  • Viral Proteins / genetics
  • Viral Proteins / physiology*

Substances

  • Glycoproteins
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Recombinant Proteins
  • Viral Proteins
  • Receptor Protein-Tyrosine Kinases
  • axl receptor tyrosine kinase