Synthesis of phosphorothioate oligonucleotide-peptide conjugates by solid phase fragment condensation

Irmina Diala; Akira Osada; Shinji Maruoka; Takashi Imanisi; Satoshi Murao; Taishi Ato; Hideki Ohba; Masayuki Fujii

doi:10.1016/j.bmcl.2007.09.101

Synthesis of phosphorothioate oligonucleotide-peptide conjugates by solid phase fragment condensation

Bioorg Med Chem Lett. 2007 Dec 1;17(23):6576-8. doi: 10.1016/j.bmcl.2007.09.101. Epub 2007 Oct 5.

Authors

Irmina Diala¹, Akira Osada, Shinji Maruoka, Takashi Imanisi, Satoshi Murao, Taishi Ato, Hideki Ohba, Masayuki Fujii

Affiliation

¹ School of Humanity Oriented Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.

PMID: 17942304
DOI: 10.1016/j.bmcl.2007.09.101

Abstract

Phosphorothioate oligonucleotide-peptide conjugates were synthesized by solid phase fragment condensation (SPFC). Arginine rich peptides could be successfully conjugated in 2.8-13.4% isolated yields. All the products were fully characterized by reversed phase HPLC and MALDI-TOF-MS to give satisfactory results.

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Cations
Chromatography, High Pressure Liquid
Humans
Molecular Sequence Data
Oligonucleotides, Antisense / chemical synthesis
Oligonucleotides, Antisense / pharmacology
Peptide Fragments / analysis
Peptide Fragments / chemical synthesis*
Phosphorothioate Oligonucleotides / analysis
Phosphorothioate Oligonucleotides / chemical synthesis*
Phosphorothioate Oligonucleotides / pharmacology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Telomerase / antagonists & inhibitors

Substances

Cations
Oligonucleotides, Antisense
Peptide Fragments
Phosphorothioate Oligonucleotides
Telomerase