The aim of this study was to evaluate the reliability of isokinetic and isometric assessments of the knee extensor and the flexor muscle function using the Con-Trex isokinetic dynamometer. Thirty healthy subjects (15 males, 15 females) were tested and retested 7 days later for maximal strength (isokinetic peak torque, work, power and angle of peak torque as well as isometric maximal voluntary contraction torque and rate of torque development) and fatigue (per cent loss and linear slope of torque and work across a series of 20 contractions). For both the knee extensor and the flexor muscle groups, all strength data - except angle of peak torque - demonstrated moderate-to-high reliability, with intraclass correlation coefficients (ICC) higher than 0.86. The highest reliability was observed for concentric peak torque of the knee extensor muscles (ICC = 0.99). Test-retest reliability of fatigue variables was moderate for the knee extensor (ICC range 0.84-0.89) and insufficient-to-moderate for the knee flexor muscles (ICC range 0.78-0.81). The more reliable index of muscle fatigue was the linear slope of the decline in work output. These findings establish the reliability of isokinetic and isometric measurements using the Con-Trex machine.