Quantification of quantum dots in HUVECs by confocal laser scanning microscopy

Abstract

Quantum dots (QDs) are emerged as a new class of fluorescent probes for many biological and biomedical applications. Comparing with conventional fluorescent probes, they have substantial advantages such as bright fluorescence, narrow emission, broad excitation band and high photostability. However, little is known about the toxicity of nanoscale particles to biological systems. In this study, the interaction between 3-Mercaptopropionic acid capped CdTe QDs and HUVECs was studied quantitatively in vitro. Fluorescent intensity of QDs in cells was measured by confocal fluorescence laser scanning microscopy. The results showed that the amount of QDs absorbed by cells is dependent on concentration and incubation time. Further, the viability of cells incubated with QDs was investigated using MTT assay. Dramatic dose-dependent decrease in cellular viability was observed.