Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression

J Gen Virol. 2007 Nov;88(Pt 11):2935-40. doi: 10.1099/vir.0.83019-0.

Abstract

The cellular protein human Daxx (hDaxx), a component of nuclear domain 10 structures, is known to mediate transcriptional repression of human cytomegalovirus immediate-early (IE) gene expression upon infection of permissive cell types, at least in part, by regulation of chromatin structure around the major IE promoter (MIEP). As it is now clear that differentiation-dependent regulation of the MIEP also plays a pivotal role in the control of latency and reactivation, we asked whether hDaxx-mediated repression is involved in differentiation-dependent MIEP regulation. We show that downregulation of hDaxx by using small interfering RNA technology in undifferentiated NT2D1 cells does not permit expression of viral IE genes, nor does it result in changes in chromatin structure around the MIEP. Viral IE gene expression is only observed upon cellular differentiation, suggesting little involvement of hDaxx in the regulation of the viral MIEP in undifferentiated cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / physiology*
  • Antigens, Viral / biosynthesis
  • Cell Line
  • Chromatin / metabolism
  • Cytomegalovirus / physiology*
  • Gene Expression Regulation, Viral*
  • Humans
  • Immediate-Early Proteins / biosynthesis
  • Nuclear Proteins / genetics
  • Nuclear Proteins / physiology*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Virus Activation / genetics
  • Virus Activation / physiology
  • Virus Latency / genetics
  • Virus Latency / physiology

Substances

  • Adaptor Proteins, Signal Transducing
  • Antigens, Viral
  • Chromatin
  • DAXX protein, human
  • Immediate-Early Proteins
  • Nuclear Proteins
  • RNA, Small Interfering
  • immediate-early proteins, cytomegalovirus