Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages

J Gen Virol. 2007 Nov;88(Pt 11):3007-12. doi: 10.1099/vir.0.83042-0.

Abstract

Classical swine fever virus (CSFV)-macrophage interactions during infection were analysed by examining macrophage transcriptional responses via microarray. Eleven genes had increased mRNA levels (>2.5-fold, P<0.05) in infected cell cultures, including arginase-1, an inhibitor of nitric oxide production, phosphoinositide 3-kinase, chemokine receptor 4 and interleukin-1beta. Lower levels of nitric oxide and increased arginase activity were found in CSFV-infected macrophages. These changes in gene expression in macrophages suggest viral modulation of host expression to suppress nitric oxide production.

MeSH terms

  • Animals
  • Arginase / analysis
  • Arginase / genetics
  • Cells, Cultured
  • Classical Swine Fever Virus / immunology*
  • Gene Expression Profiling
  • Gene Expression Regulation*
  • Macrophages / immunology*
  • Macrophages / virology*
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide / biosynthesis
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Swine

Substances

  • RNA, Messenger
  • Nitric Oxide
  • Arginase