Rapid identification and specific quantification of Staphylococcus epidermidis by 5' nuclease real-time polymerase chain reaction with a minor groove binder probe

Diagn Microbiol Infect Dis. 2008 Feb;60(2):217-9. doi: 10.1016/j.diagmicrobio.2007.08.009. Epub 2007 Oct 22.

Abstract

A species-specific quantitative detection method involving 5' nuclease real-time polymerase chain reaction using a minor groove binder probe that was designed from the sodA gene was developed for Staphylococcus epidermidis. This method distinguished S. epidermidis from other staphylococci and specifically quantified the bacterium. This study shows that the method is useful for the identification and quantitative detection of S. epidermidis.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / genetics
  • Colony Count, Microbial / methods*
  • Humans
  • Oligonucleotide Probes / genetics*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcus epidermidis / genetics
  • Staphylococcus epidermidis / isolation & purification*
  • Superoxide Dismutase / genetics

Substances

  • Bacterial Proteins
  • Oligonucleotide Probes
  • SodA protein, Bacteria
  • Superoxide Dismutase