Enhanced antitumor effects by the coculture of allotumor RNA-pulsed dendritic cells with autologous cytokine-induced killer cells on hormone-refractory prostate cancer

Cancer Invest. 2007;25(7):527-34. doi: 10.1080/07357900701511789.


In this study, we evaluated antitumor effects of allotumour RNA-transfected dendritic cells (DCs) cocultured with autologous cytokine-induced killer cells (CIKs) on hormone-refractory prostate cancer. The cocultured cells enhanced prostate cancer cytolysis from 26% (CIKs-induced cytolysis) to 80.8%. They also increased the productions of CD4(+) Th1 (IFN-γ(+)IL-4(-), 55.52%) and CD8(+) T (IFN-γ(+), 69.59%) cells determined by intracellular cytokines IFN-γ /IL-4 staining and reduced the rate of CD4(+) CD25(+) cells from 18.72% (in CIKs) to 9.72%. The cocultured cells significantly inhibited tumor growth in SCID mouse and induced cancer cells necrosis and apoptosis. Our study indicates that tumor RNA-pulsed DCs cocultured with autologous CIKs significantly enhance antitumor immunity, which can be induced by increased CD4(+) Th1 and CD8(+) T cells and decreased CD4(+)CD25(+) regulatory T (T(reg)) cells. This provides a potential immunotherapy strategy for HRPC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androgen Antagonists / pharmacology*
  • Animals
  • Cell Line, Tumor
  • Cell Proliferation
  • Coculture Techniques
  • Cytokine-Induced Killer Cells / immunology*
  • Cytotoxicity, Immunologic
  • Dendritic Cells / immunology*
  • Drug Resistance, Neoplasm*
  • Humans
  • Male
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Prostatic Neoplasms / therapy*
  • RNA / immunology*
  • Transfection


  • Androgen Antagonists
  • RNA