Dynamic regulation of fluorescent proteins from a single species of coral

Mar Biotechnol (NY). 2007 Nov-Dec;9(6):733-46. doi: 10.1007/s10126-007-9025-1. Epub 2007 Oct 23.

Abstract

To gain a better understanding of the natural function of fluorescent proteins, we have undertaken quantitative analyses of these proteins in a single species of coral, Montastraea cavernosa, residing around Turneffe atoll, on the Belizean Barrier Reef. We identified at least 10 members of a fluorescent protein family in this species, which consist of 4 distinct spectral classes. As much as a 10-fold change in the overall expression of fluorescent proteins was observed from specimen to specimen, suggesting that fluorescent proteins are dynamically regulated in response to environmental or physiological conditions. We found that the expression of some proteins was inversely correlated with depth, and that groups of proteins were coordinately expressed. There was no relationship between the expression of fluorescent proteins and the natural coloration of the Montastraea cavernosa specimens in this study. These findings have implications for current hypotheses regarding the properties and natural function of fluorescent proteins.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anthozoa / chemistry
  • Anthozoa / genetics
  • Anthozoa / physiology*
  • Base Sequence
  • DNA Primers / chemistry
  • Environment*
  • Gene Expression Profiling / veterinary
  • Gene Expression Regulation / physiology*
  • Luminescent Proteins / biosynthesis
  • Luminescent Proteins / genetics
  • Luminescent Proteins / physiology*
  • Molecular Sequence Data
  • Phylogeny
  • Pigmentation
  • RNA, Ribosomal, 18S / analysis
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sensitivity and Specificity
  • Statistics as Topic
  • Tubulin / analysis
  • Tubulin / biosynthesis

Substances

  • DNA Primers
  • Luminescent Proteins
  • RNA, Ribosomal, 18S
  • Tubulin

Associated data

  • GENBANK/EU035527
  • GENBANK/EU035528
  • GENBANK/EU035529
  • GENBANK/EU035530
  • GENBANK/EU035531
  • GENBANK/EU035532
  • GENBANK/EU035533
  • GENBANK/EU035534
  • GENBANK/EU035535
  • GENBANK/EU035536