Plant pathogen recognition mediated by promoter activation of the pepper Bs3 resistance gene

Science. 2007 Oct 26;318(5850):645-8. doi: 10.1126/science.1144958.

Abstract

Plant disease resistance (R) proteins recognize matching pathogen avirulence proteins. Alleles of the pepper R gene Bs3 mediate recognition of the Xanthomonas campestris pv. vesicatoria (Xcv) type III effector protein AvrBs3 and its deletion derivative AvrBs3Deltarep16. Pepper Bs3 and its allelic variant Bs3-E encode flavin monooxygenases with a previously unknown structure and are transcriptionally activated by the Xcv effector proteins AvrBs3 and AvrBs3Deltarep16, respectively. We found that recognition specificity resides in the Bs3 and Bs3-E promoters and is determined by binding of AvrBs3 or AvrBs3Deltarep16 to a defined promoter region. Our data suggest a recognition mechanism in which the Avr protein binds and activates the promoter of the cognate R gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Base Sequence
  • Capsicum / genetics*
  • Capsicum / microbiology*
  • Chromosomes, Artificial, Bacterial
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / genetics*
  • Molecular Sequence Data
  • Plant Diseases / immunology
  • Plant Diseases / microbiology
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Promoter Regions, Genetic*
  • Tobacco / genetics
  • Transcription, Genetic
  • Transformation, Genetic
  • Xanthomonas campestris / genetics
  • Xanthomonas campestris / metabolism
  • Xanthomonas campestris / pathogenicity*

Substances

  • Bacterial Proteins
  • Plant Proteins
  • Mixed Function Oxygenases

Associated data

  • GENBANK/EU078683
  • GENBANK/EU078684