Establishment and characterization of an Epstein-Barr virus (EBC)-negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt's lymphoma

Biomedicine. 1975 Jul;22(4):276-84.


An Epstein-Barr virus (EBV)-negative lymphoblastoid cell line (LCL), BJA-B, was established from an African Burkitt's lymphoma (BL) which contained no detectable EBV DNA and did not express the EBV specified antigen EBNA, BJA-B cells grow in typically large, flat clumps. All carry surface-bound immunoglobulins, a B lymphocyte marker, and do not form rosettes with sheep erythrocytes. After infection of BJA-B cells by EBV the infected cells may produce either EBV-determined nuclear antigen (EBNA) or both EBNA and early antigen (EA), depending on the strain of EBV. The homogeneity of the BJA-B cell population with respect to immunological and isoenzyme markers and size suggests a clonal origin of the line. BJA-B is the first EBV-negative LCL established from an African Burkitt's lymphoma and demonstrates that an EBV-independent continuous B cell line can be established "in vitro" from other than leukemia or myeloma cells.

MeSH terms

  • Antigens, Viral / analysis
  • B-Lymphocytes
  • Burkitt Lymphoma / immunology
  • Burkitt Lymphoma / microbiology*
  • Cell Line*
  • Child, Preschool
  • Chromosomes
  • Erythrocytes / immunology
  • Female
  • Glucosephosphate Dehydrogenase / analysis
  • Herpesvirus 4, Human / immunology*
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Immunoglobulin Fragments / analysis
  • Isoenzymes / analysis
  • Lymphocyte Activation
  • Microscopy, Electron, Scanning
  • Phosphoglycerate Mutase / analysis


  • Antigens, Viral
  • Immunoglobulin Fragments
  • Isoenzymes
  • Glucosephosphate Dehydrogenase
  • Phosphoglycerate Mutase