Expression patterns of microRNAs 155 and 451 during normal human erythropoiesis

Biochem Biophys Res Commun. 2007 Dec 21;364(3):509-14. doi: 10.1016/j.bbrc.2007.10.077. Epub 2007 Oct 23.


To clarify the roles of microRNAs (miRNAs) in erythropoiesis, the expression of miR-155, miR-221, miR-223, and miR-451 were analyzed during the differentiation of purified normal human erythroid progenitors in a liquid culture system. Cells increased almost 500-fold in a number, and differentiated to benzidine-positive mature erythroblasts. Analyses of miRNA expression using the quantitative real-time polymerase chain reaction showed that the expression level of miR-155 decreased about 200-fold, and that the expression of miR-451 increased about 270-fold during 12 days of cultures. A moderate down-regulation of miR-221 and miR-223 was observed. MiR-451 was expressed in red blood cells about 10(4)-fold more than in granulocytes, obtained from normal human peripheral blood. These observations suggest that miR-155 and miR-451 are key molecules for normal erythroid differentiation, and that quantitative assays of the two miRNAs may be a relevant method for analyzing pathological erythropoiesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cells, Cultured
  • Erythrocytes / cytology*
  • Erythrocytes / physiology*
  • Erythropoiesis / genetics*
  • Gene Expression Regulation / genetics
  • Humans
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / physiology*
  • MicroRNAs / genetics*


  • MIRN155 microRNA, human
  • MicroRNAs