Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 52 (6), 1140-53

Overexpression of a Chromatin Architecture-Controlling AT-hook Protein Extends Leaf Longevity and Increases the Post-Harvest Storage Life of Plants

Affiliations

Overexpression of a Chromatin Architecture-Controlling AT-hook Protein Extends Leaf Longevity and Increases the Post-Harvest Storage Life of Plants

Pyung Ok Lim et al. Plant J.

Abstract

Leaf senescence is the final stage of leaf development and is finely regulated via a complex genetic regulatory network incorporating both developmental and environmental factors. In an effort to identify negative regulators of leaf senescence, we screened activation-tagged Arabidopsis lines for mutants that exhibit a delayed leaf senescence phenotype. One of the mutants (ore7-1D) showed a highly significant delay of leaf senescence in the heterozygous state, leading to at least a twofold increase in leaf longevity. The activated gene (ORE7/ESC) encoded a protein with an AT-hook DNA-binding motif; such proteins are known to co-regulate transcription of genes through modification of chromatin architecture. We showed that ORE7/ESC, in addition to binding to a plant AT-rich DNA fragment, could also modify the chromatin architecture, as illustrated by an altered distribution of a histone-GFP fusion protein in the nucleus of the mutant. Globally altered gene expression, shown by microarray analysis, also indicated that activation of ORE7/ESC results in a younger condition in the mutant leaves. We propose that ectopically expressed ORE7/ESC is negatively regulating leaf senescence and suggest that the resulting chromatin alteration may have a role in controlling leaf longevity. Interestingly, activation of ORE7/ESC also led to a highly extended post-harvest storage life.

Similar articles

See all similar articles

Cited by 38 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback