Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays

Chem Commun (Camb). 2007 Nov 21;(43):4459-61. doi: 10.1039/b709817k. Epub 2007 Aug 22.

Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA-microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biological Assay / methods
  • Biosensing Techniques / methods
  • DNA / chemistry*
  • DNA / metabolism
  • DNA Ligases / chemistry*
  • DNA Ligases / metabolism
  • DNA Probes / chemistry
  • DNA-Directed DNA Polymerase / chemistry*
  • DNA-Directed DNA Polymerase / metabolism
  • Gels / chemistry*
  • Molecular Sequence Data

Substances

  • DNA Probes
  • Gels
  • DNA
  • DNA-Directed DNA Polymerase
  • DNA Ligases