This study was conducted to develop a testing protocol which would determine the extent of upper-body power output decrements in subjects following weight loss. Five athletes who had trained via upper-body exercise performed a 6-minute variable intensity arm crank test on an isokinetic ergometer before and after a 3-day, 4.5% body weight loss. Blood samples were drawn from a forearm vein pre- and 1, 3, and 5 min post-arm cranking for assessment of lactate, pH, hemoglobin, and hematocrit. The work performed pre-weight loss was significantly (paired t-test, p less than 0.05) greater than that performed post-weight loss. Repeated measures ANOVA yielded no significant differences in blood variables; however, pre-weight loss lactate values were higher and hemoglobin, hematocrit, and pH values were lower than post-weight loss values. It was concluded that a 4.5% body weight reduction resulted in performance decrements during this arm crank test. Survey information obtained from collegiate wrestlers (n = 14) subsequently tested under this protocol indicates the physical demands of this test approximate the physical demands of actual wrestling competition. It would therefore be appropriate to use this protocol during future testing of wrestlers in weight loss studies.