Abstract
Under standard culture conditions, tumor cells are exposed to 20% O(2), whereas the mean tumor oxygen levels within the tumor are much lower. We demonstrate, using low-passaged human tumor cell cultures established from glioma, that a reduction in the oxygen level in these cell cultures dramatically increases the percentage of CD133 expressing cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AC133 Antigen
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Antigens, CD / genetics
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Antigens, CD / metabolism*
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Cell Hypoxia
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Flow Cytometry
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Gene Expression Regulation, Neoplastic / drug effects
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Glioma / metabolism
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Glioma / pathology
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Glycoproteins / genetics
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Glycoproteins / metabolism*
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Humans
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Immunohistochemistry
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Oxygen / pharmacology*
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Peptides / genetics
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Peptides / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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AC133 Antigen
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Antigens, CD
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Glycoproteins
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PROM1 protein, human
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Peptides
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Oxygen