Influence of oxygen tension on CD133 phenotype in human glioma cell cultures

Cancer Lett. 2007 Dec 18;258(2):286-90. doi: 10.1016/j.canlet.2007.09.012. Epub 2007 Oct 30.

Abstract

Under standard culture conditions, tumor cells are exposed to 20% O(2), whereas the mean tumor oxygen levels within the tumor are much lower. We demonstrate, using low-passaged human tumor cell cultures established from glioma, that a reduction in the oxygen level in these cell cultures dramatically increases the percentage of CD133 expressing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Antigens, CD / genetics
  • Antigens, CD / metabolism*
  • Cell Hypoxia
  • Cell Line, Tumor
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • Gene Expression Regulation, Neoplastic / drug effects
  • Glioma / metabolism
  • Glioma / pathology
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Humans
  • Immunohistochemistry
  • Oxygen / pharmacology*
  • Peptides / genetics
  • Peptides / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • AC133 Antigen
  • Antigens, CD
  • Glycoproteins
  • PROM1 protein, human
  • Peptides
  • Oxygen