High-performance liquid chromatographic separation of the enantiomers of hydroxychloroquine and its major metabolites in biological fluids using an alpha 1-acid glycoprotein stationary phase

J Chromatogr. 1991 Sep 18;570(1):119-27. doi: 10.1016/0378-4347(91)80206-r.


An enantioselective two-stage off-line assay has been developed for the analysis of hydroxychloroquine and its three major metabolites in biological fluids. The first non-stereoselective stage of the assay (PRP-1 column) separates and quantitates parent drug and metabolites. Fractions containing hydroxychloroquine and each of the metabolites are collected manually, evaporated, reconstituted in mobile phase and re-injected onto an alpha 1-acid glycoprotein column to separate and determine proportions of individual enantiomers. Preliminary results from patients samples indicate that the disposition of hydroxychloroquine and its major metabolites is enantioselective. p6

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Body Fluids / chemistry*
  • Chromatography, High Pressure Liquid
  • Humans
  • Hydroxychloroquine / metabolism*
  • Orosomucoid*
  • Stereoisomerism


  • Orosomucoid
  • Hydroxychloroquine