Halorubrum ejinorense sp. nov., isolated from Lake Ejinor, Inner Mongolia, China

Int J Syst Evol Microbiol. 2007 Nov;57(Pt 11):2538-2542. doi: 10.1099/ijs.0.65241-0.

Abstract

A novel halophilic archaeon, strain EJ-32T, was isolated from water from Lake Ejinor in Inner Mongolia, China. The taxonomy of strain EJ-32T was studied by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarities, strain EJ-32T was shown to be phylogenetically related to Halorubrum coriense (97.9%), Halorubrum trapanicum (97.9%), Halorubrum sodomense (97.8%), Halorubrum tebenquichense (97.8%), Halorubrum xinjiangense (97.6%), Halorubrum terrestre (97.4%), Halorubrum distributum (97.1%) and Halorubrum saccharovorum (96.4%). Strain EJ-32T was found to be neutrophilic, non-motile and Gram-negative. It grew in medium containing saturation concentrations of NaCl and did not require magnesium for optimal growth. The G+C content of the DNA is 64.0 mol%. Values for DNA-DNA hybridization with respect to phylogenetically related Halorubrum species were <or=49%, indicating that EJ-32T constitutes a different genospecies. The data show that strain EJ-32T represents a novel species of the genus Halorubrum, for which the name Halorubrum ejinorense sp. nov. is proposed. The type strain is EJ-32T (=CECT 7194T=CGMCC 1.6782T=JCM 14265T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • China
  • DNA, Archaeal / analysis
  • DNA, Ribosomal / analysis
  • Fresh Water / microbiology*
  • Genes, rRNA
  • Genotype
  • Halobacteriaceae / classification*
  • Halobacteriaceae / genetics*
  • Halobacteriaceae / isolation & purification
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sodium Chloride / metabolism*
  • Species Specificity

Substances

  • DNA, Archaeal
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Sodium Chloride

Associated data

  • GENBANK/AM491830