In vitro activity of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin against Propionibacterium acnes

J Microbiol. 2007 Oct;45(5):473-7.

Abstract

The in vitro antibacterial activity against antibiotic-resistant Propionibacterium acnes of kaempferol isolated from the Impatiens balsamina alone and in combination with erythromycin or clindamycin antibiotics was investigated. The antibiotic combination effect against antibiotic-resistant P. acnes was studied by checkerboard test. Kaempferol and quercetin demonstrated antibacterial activities against P. acnes. Minimum inhibitory concentrations (MICs) for both compounds were < or =32 ug/ml and < or =64 ug/ml for clindamycin-sensitive and-resistant P. acnes, respectively. The four combination formulations (kaempferol and either erythromycin or clindamycin; quercetin and either erythromycin or clindamycin) exhibited a synergic inhibition of P. acnes growth. The combination of kaempferol with quercetin showed an indifferent effect. The combination of clindamycin with kaempferol or quercetin showed a greater synergic effect than that of erythromycin with kaempferol or quercetin. Thus, these combinations demonstrated the potential to treat acne.

MeSH terms

  • Ampicillin / pharmacology
  • Chloramphenicol / pharmacology
  • Clindamycin / pharmacology*
  • Drug Resistance, Bacterial
  • Drug Synergism
  • Erythromycin / pharmacology*
  • Impatiens / chemistry*
  • Kaempferols / isolation & purification*
  • Kaempferols / pharmacology*
  • Microbial Sensitivity Tests
  • Propionibacterium acnes / drug effects*
  • Propionibacterium acnes / growth & development
  • Tetracycline / pharmacology

Substances

  • Kaempferols
  • Clindamycin
  • Erythromycin
  • Chloramphenicol
  • kaempferol
  • Ampicillin
  • Tetracycline