CD24 induces localization of beta1 integrin to lipid raft domains

Biochem Biophys Res Commun. 2008 Jan 4;365(1):35-41. doi: 10.1016/j.bbrc.2007.10.139. Epub 2007 Nov 1.


The expression of the glycosyl phosphatidylinositol (GPI)-anchored protein CD24 correlates with poor prognosis in a variety of carcinomas. However, little is known about the cellular mechanisms of the CD24-mediated effects. In this study, we present evidence that CD24 affects the lateral localization of beta1 integrin. Using stably CD24-transfected A125 and MDA-MB-435S carcinoma cells we show that CD24 augments beta1-dependent cell motility and stimulates transmigration and invasion across a monolayer of endothelial cells. Furthermore, as demonstrated by sucrose density gradient centrifugation and Western Blot analysis, CD24 recruits beta1 integrin into lipid raft domains. We suggest that CD24 acts as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / metabolism
  • CD24 Antigen / metabolism*
  • Cell Line, Tumor
  • Female
  • Humans
  • Integrin beta1 / analysis*
  • Integrin beta1 / immunology
  • Lung Neoplasms / metabolism
  • Membrane Microdomains / metabolism*
  • Protein Subunits / analysis
  • Transfection


  • CD24 Antigen
  • CD24 protein, human
  • Integrin beta1
  • Protein Subunits