Bicaudal-C Recruits CCR4-NOT Deadenylase to Target mRNAs and Regulates Oogenesis, Cytoskeletal Organization, and Its Own Expression

Dev Cell. 2007 Nov;13(5):691-704. doi: 10.1016/j.devcel.2007.10.002.

Abstract

Bicaudal-C (Bic-C) encodes an RNA-binding protein required maternally for patterning the Drosophila embryo. We identified a set of mRNAs that associate with Bic-C in ovarian ribonucleoprotein complexes. These mRNAs are enriched for mRNAs that function in oogenesis and in cytoskeletal regulation, and include Bic-C RNA itself. Bic-C binds specific segments of the Bic-C 5' untranslated region and negatively regulates its own expression by binding directly to NOT3/5, a component of the CCR4 core deadenylase complex, thereby promoting deadenylation. Bic-C overexpression induces premature cytoplasmic-streaming, a posterior-group phenotype, defects in Oskar and Kinesin heavy chain:betaGal localization as well as dorsal-appendage defects. These phenotypes are largely reciprocal to those of Bic-C mutants, and they affect cellular processes that Bic-C-associated mRNAs are known, or predicted, to regulate. We conclude that Bic-C regulates expression of specific germline mRNAs by controlling their poly(A)-tail length.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Animals
  • Biological Transport, Active
  • Body Patterning
  • Cytoplasm / metabolism
  • Cytoskeleton / physiology*
  • Drosophila / embryology
  • Drosophila / physiology*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Female
  • Mutation
  • Oogenesis
  • Poly A / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / physiology*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / physiology*
  • Ribonucleases / genetics
  • Ribonucleases / physiology*

Substances

  • 5' Untranslated Regions
  • BicC protein, Drosophila
  • Drosophila Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Poly A
  • CCR4 protein, Drosophila
  • Ribonucleases