Characterization of agonist-induced vasoconstriction in mouse pulmonary artery

Am J Physiol Heart Circ Physiol. 2008 Jan;294(1):H220-8. doi: 10.1152/ajpheart.00968.2007. Epub 2007 Nov 2.


In recent years, transgenic mouse models have been developed to examine the underlying cellular and molecular mechanisms of lung disease and pulmonary vascular disease, such as asthma, pulmonary thromboembolic disease, and pulmonary hypertension. However, there has not been systematic characterization of the basic physiological pulmonary vascular reactivity in normal and transgenic mice. This represents an intellectual "gap", since it is important to characterize basic murine pulmonary vascular reactivity in response to various contractile and relaxant factors to which the pulmonary vasculature is exposed under physiological conditions. The present study evaluates excitation- and pharmacomechanical-contraction coupling in pulmonary arteries (PA) isolated from wild-type BALB/c mice. We demonstrate that both pharmaco- and electromechanical coupling mechanisms exist in mice PA. These arteries are also reactive to stimulation by alpha(1)-adrenergic agonists, serotonin, endothelin-1, vasopressin, and U-46619 (a thromboxane A(2) analog). We conclude that the basic vascular responsiveness of mouse PA is similar to those observed in PA of other species, including rat, pig, and human, albeit on a different scale and to varying amplitudes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
  • 4-Aminopyridine / pharmacology
  • Acetylcholine / pharmacology
  • Adrenergic alpha-Agonists / pharmacology
  • Animals
  • Calcium / metabolism
  • Dose-Response Relationship, Drug
  • Endothelin-1 / metabolism
  • In Vitro Techniques
  • Male
  • Membrane Potentials / drug effects
  • Mice
  • Mice, Inbred BALB C
  • Phenylephrine / pharmacology
  • Potassium / metabolism
  • Potassium Channel Blockers / pharmacology
  • Potassium Channels / drug effects
  • Potassium Channels / metabolism
  • Pulmonary Artery / cytology
  • Pulmonary Artery / drug effects*
  • Pulmonary Artery / metabolism*
  • Serotonin / metabolism
  • Species Specificity
  • Stress, Mechanical
  • Thrombin / pharmacology
  • Vasoconstriction / drug effects*
  • Vasoconstrictor Agents / metabolism*
  • Vasoconstrictor Agents / pharmacology*
  • Vasodilator Agents / pharmacology
  • Vasopressins / metabolism


  • Adrenergic alpha-Agonists
  • Endothelin-1
  • Potassium Channel Blockers
  • Potassium Channels
  • Vasoconstrictor Agents
  • Vasodilator Agents
  • Vasopressins
  • Phenylephrine
  • Serotonin
  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
  • 4-Aminopyridine
  • Thrombin
  • Acetylcholine
  • Potassium
  • Calcium