Two-color immunofluorescence and flow cytometric analysis of peripheral blood lymphocyte subsets in Caucasian and Japanese healthy subjects

Jpn J Med. Nov-Dec 1991;30(6):509-15. doi: 10.2169/internalmedicine1962.30.509.


Two-color immunofluorescence using multiparameter flow cytometry was employed to examine the antigenic characteristics of peripheral blood lymphocytes in whole blood of healthy Caucasians and Japanese. The CD4/CD8 ratio in Japanese was significantly decreased compared with that in Caucasians, because of the increased number of CD8+ cells. Although the proportions of suppressor-inducer T cells (CD4+, Leu-8+) and helper-inducer T cells (naive T cells) (CD4+, CD45RA-) were low in Japanese subjects, there were no differences in the absolute numbers of suppressor-inducer T cells and helper-inducer T cells (naive T cells) in circulation. The level of activated T cells in Japanese was similar to that in Caucasians. NK cells, CD57+, CD8+ cells and CD57+, CD3+ cells were high in Japanese. Regarding B cell subsets, CD5+ B cells and activated B cells remained unchanged. However, there were slight differences in Leu-8+ B cells and Fc epsilon R+ B cells (CD20+, CD23+) between the two groups. Thus, a differing influence of racial and environmental background between healthy Caucasians and healthy Japanese on human lymphocyte subsets is present in the lymphocyte immunophenotype.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antigens, Differentiation
  • Asian Continental Ancestry Group
  • CD4-CD8 Ratio
  • European Continental Ancestry Group
  • Female
  • Flow Cytometry
  • Fluorescent Antibody Technique
  • Humans
  • Japan
  • Lymphocyte Subsets / cytology*
  • Lymphocyte Subsets / immunology
  • Male
  • Middle Aged


  • Antigens, Differentiation