Evaluation of anti-inflammatory activity of alcoholic extract from Viola tricolor

Rev Med Chir Soc Med Nat Iasi. 2007 Apr-Jun;111(2):525-9.


Aim: Evaluation of anti-inflammatory actions of Viola tricolor aerial parts tincture.

Material and method: The anti-inflammatory activity of the tincture from Viola tricolor aerial parts was tested in acute inflammation induced with oil of turpentine (i.m. 0.6 ml/100 g b.w.) in male Wistar rats. The results were compared with those from a positive control group with experimental inflammation and with those of a group treated with diclofenac (30 mg/100 g b.w.). We tested the effects by measuring total leukocyte count and differential leukocyte count, a test of in vitro phagocytosis and by the evaluation of nitric oxide (NO) synthesis measuring the metabolites (nitrites and nitrates).

Results: Viola tricolor extract (50 mg tincture/100 g b.w.) significantly reduced polymorphonuclear leukocytes and monocytes percentages and the activation of circulating phagocytes. Nitric oxide synthesis had a slight decrease.

Conclusion: Viola tricolor extract had anti-inflammatory effect on bone marrow acute phase response.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
  • Diclofenac / therapeutic use
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Inflammation / chemically induced
  • Inflammation / drug therapy*
  • Irritants / adverse effects
  • Leukocyte Count
  • Male
  • Nitric Oxide / metabolism
  • Phagocytosis / drug effects
  • Phytotherapy*
  • Plant Extracts / therapeutic use*
  • Rats
  • Rats, Wistar
  • Viola*


  • Anti-Inflammatory Agents, Non-Steroidal
  • Irritants
  • Plant Extracts
  • Diclofenac
  • Nitric Oxide