Methods in high-resolution, array-based comparative genomic hybridization

Methods Mol Biol. 2007;381:189-211. doi: 10.1007/978-1-59745-303-5_9.


A method of high resolution, array-based comparative genomic hybridization is described for the mapping of copy-number changes associated with chromosomal amplifications, deletions, and translocations. The method involves the design of whole-genome or targeted, fine-tiling arrays for synthesis on a high-density digital microarray-synthesis platform. The arrays can span entire eukaryotic genomes or be targeted to specific chromosomal regions for high-resolution identification of copy-number changes and the corresponding breakpoint locations. The methods described include the bioinformatics required for array design, and the protocols for DNA fragmentation, dual-color labeling, microarray hybridization, and array scanning. The processes for data extraction, normalization, and segmentation analysis are also described.

MeSH terms

  • Animals
  • Buffers
  • Cell Line, Tumor
  • Computational Biology
  • Electrophoresis
  • Genome*
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Reference Standards
  • Software


  • Buffers