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Comparative Study
, 116 (2), 297-303

The Novel Gene Ny-1 on Potato Chromosome IX Confers Hypersensitive Resistance to Potato Virus Y and Is an Alternative to Ry Genes in Potato Breeding for PVY Resistance

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Comparative Study

The Novel Gene Ny-1 on Potato Chromosome IX Confers Hypersensitive Resistance to Potato Virus Y and Is an Alternative to Ry Genes in Potato Breeding for PVY Resistance

K Szajko et al. Theor Appl Genet.

Abstract

Hypersensitive resistance (HR) is an efficient defense strategy in plants that restricts pathogen growth and can be activated during host as well as non-host interactions. HR involves programmed cell death and manifests itself in tissue collapse at the site of pathogen attack. A novel hypersensitivity gene, Ny-1, for resistance to Potato virus Y (PVY) was revealed in potato cultivar Rywal. This is the first gene that confers HR in potato plants both to common and necrotic strains of PVY. The locus Ny-1 mapped on the short arm of potato chromosome IX, where various resistance genes are clustered in Solanaceous genomes. Expression of HR was temperature-dependent in cv. Rywal. Strains PVYO and PVYN, including subgroups PVYNW and PVYNTN, were effectively localized when plants were grown at 20 degrees C. At 28 degrees C, plants were systemically infected but no symptoms were observed. In field trials, PVY was restricted to the inoculated leaves and PVY-free tubers were produced. Therefore, the gene Ny-1 can be useful for potato breeding as an alternative donor of PVY resistance, because it is efficacious in practice-like resistance conferred by Ry genes.

Figures

Fig. 1
Fig. 1
Response of cv. Rywal plants to infection with PVY0LW, PVYNNy, PVYNW or PVYNTNBo. The pictures of detached leaves incubated at 20 or 28°C were taken 6 days post-inoculation. Experiments were repeated twice with similar results
Fig. 2
Fig. 2
Patterns of amplified DNA of resistant parent cv. Rywal (lanes 1, 3, 5 and 7) and susceptible parent Accent (lanes 2, 4, 6 and 8), indicating the ISSR marker UBC8951200 (lane 1), the STS marker SC8951139 (lane 3), the SCAR marker GP41443 (lane 5) and the COSII marker C2_At3g16840 after digestion with TaqI (lanes 7 and 8). Arrows point to the marker products that were scored. Lanes M contain the 250-bp (upper) and 100-bp (lower) DNA ladders as molecular size markers
Fig. 3
Fig. 3
Potato linkage group IX of PVY resistant parent cv. Rywal, including the position of the locus Ny-1. The tetraploid population obtained from a cross between potato cultivars Rywal and Accent was used for the mapping

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References

    1. {'text': '', 'index': 1, 'ids': [{'type': 'DOI', 'value': '10.1111/j.1365-3059.1986.tb02050.x', 'is_inner': False, 'url': 'https://doi.org/10.1111/j.1365-3059.1986.tb02050.x'}]}
    2. Adams SE, Jones RAC, Coutts RHA (1986) Effect of temperature on potato virus X infection in potato cultivars carrying different combinations of hypersensitivity genes. Plant Pathol 35:517–526
    1. {'text': '', 'index': 1, 'ids': [{'type': 'DOI', 'value': '10.1007/BF02357646', 'is_inner': False, 'url': 'https://doi.org/10.1007/bf02357646'}]}
    2. Beczner L, Horvath J, Romhanyi I, Forster H (1984) Studies on the etiology of tuber necrotic ringspot disease in potato. Potato Res 27:339–352
    1. {'text': '', 'index': 1, 'ids': [{'type': 'DOI', 'value': '10.1007/s001220050400', 'is_inner': False, 'url': 'https://doi.org/10.1007/s001220050400'}]}
    2. Brigneti G, Garcia-Mas J, Baulcombe DC (1997) Molecular maping of the potato virus Y resistance gene Rysto in potato. Theor Appl Genet 94:198–203
    1. None
    2. Brunt AA (2001) Potyviruses. In: Loebenstein G, Berger PH, Brunt AA, Lawson RH (eds) Virus and virus-like diseases of potatoes and production of seed-potatoes. Kluwer, Dordrecht, Boston, London, pp 77–84
    1. {'text': '', 'index': 1, 'ids': [{'type': 'DOI', 'value': '10.1007/s001220100749', 'is_inner': False, 'url': 'https://doi.org/10.1007/s001220100749'}, {'type': 'PubMed', 'value': '12582672', 'is_inner': True, 'url': 'http://www.ncbi.nlm.nih.gov/pubmed/12582672/'}]}
    2. Celebi –Toprak F, Slack SA, Jahn MM (2002) A new gene, Nytbr, for hypersensitivity to Potato virus Y from Solanum tuberosum maps to chromosome IV. Theor Appl Genet 104:669–674 - PubMed

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