Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration

Biochem Biophys Res Commun. 2008 Jan 11;365(2):285-90. doi: 10.1016/j.bbrc.2007.10.166. Epub 2007 Nov 5.

Abstract

Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. Wnt signalling is mediated through cell-cell interaction and is involved in many developmental processes and cellular functions. In this study, we investigated the possible function of Wnt5a and the non-canonical Wnt pathway in human endothelial cells. We found that Wnt5a-mediated non-canonical Wnt signalling regulated endothelial cell proliferation. Blocking this pathway using antibody, siRNA or a down-stream inhibitor led to suppression of endothelial cell proliferation, migration, and monolayer wound closure. We also found that the mRNA level of Wnt5a is up-regulated when endothelial cells are treated with a cocktail of inflammatory cytokines. Our findings suggest non-canonical Wnt signalling plays a role in regulating endothelial cell growth and possibly in angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Endothelial Cells / cytology
  • Endothelial Cells / physiology*
  • Humans
  • Proto-Oncogene Proteins / metabolism*
  • Signal Transduction / physiology*
  • Wnt Proteins / metabolism*
  • Wnt-5a Protein

Substances

  • Proto-Oncogene Proteins
  • WNT5A protein, human
  • Wnt Proteins
  • Wnt-5a Protein