Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells

J Biol Chem. 2008 Jan 11;283(2):1026-33. doi: 10.1074/jbc.M707224200. Epub 2007 Nov 8.


MicroRNAs are emerging as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally linked to cellular proliferation, apoptosis, and migration. Inhibition of mir-21 in MCF-7 breast cancer cells causes reduced cell growth. Using array expression analysis of MCF-7 cells depleted of miR-21, we have identified mRNA targets of mir-21 and have shown a link between miR-21 and the p53 tumor suppressor protein. We furthermore found that the tumor suppressor protein Programmed Cell Death 4 (PDCD4) is regulated by miR-21 and demonstrated that PDCD4 is a functionally important target for miR-21 in breast cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / physiology*
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Division
  • Cell Line, Tumor
  • Female
  • Humans
  • Kinetics
  • MicroRNAs / genetics*
  • RNA, Neoplasm / genetics*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / physiology*


  • Apoptosis Regulatory Proteins
  • MicroRNAs
  • PDCD4 protein, human
  • RNA, Neoplasm
  • RNA-Binding Proteins