Abstract
Objective:
To study the effects of volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root (OSS) on proinflammatory cytokine expression and regulation in rats.
Method:
OA and LPS were injected intravenously to rats to develop acute lung injury (ALI). The rats were treated with OSS (45.19 microL kg(-1)). The pathological sections of lung tissue were prepared and observed in acute lung injury rats. The expression of nuclear factor-kappa B p65 (NF-kappaB p65), intercellar adhesion molecule CD54, and NF-kappaB p65 mRNA were determined in lung cells.
Result:
volatie oil of Schizonepeta tenuifolia Briq herb and Saposhnikovia divaricata Schischke root significantly inhibited the expression of CD54, the activation of NF-kappaB p65, and the transcription of NF-kappaB p65 mRNA.
Conclusion:
OSS can reduce the expression of CD54 and NF-kappaB p65 protein synthesis, which may be its anti-inflammatory molecular mechanisms.
MeSH terms
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Animals
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology
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Apiaceae / chemistry
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Drug Combinations
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Gene Expression Regulation / drug effects
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Immunohistochemistry
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Intercellular Adhesion Molecule-1 / biosynthesis*
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Lamiaceae / chemistry
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Lipopolysaccharides
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Male
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Oils, Volatile / isolation & purification
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Oils, Volatile / pharmacology*
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Oleic Acid
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Plant Oils / isolation & purification
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Plant Oils / pharmacology*
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Plants, Medicinal / chemistry
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Random Allocation
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Rats
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Rats, Wistar
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Respiratory Distress Syndrome / chemically induced
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Respiratory Distress Syndrome / metabolism*
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Respiratory Distress Syndrome / prevention & control
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Transcription Factor RelA / biosynthesis*
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Transcription Factor RelA / genetics
Substances
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Anti-Inflammatory Agents
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Drug Combinations
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Lipopolysaccharides
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Oils, Volatile
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Plant Oils
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RNA, Messenger
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Transcription Factor RelA
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Intercellular Adhesion Molecule-1
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Oleic Acid