Platelet gel stimulates proliferation of human dermal fibroblasts in vitro

Acta Dermatovenerol Alp Pannonica Adriat. 2007 Sep;16(3):105-10.


Background: Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing.

Objective: The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro.

Methods: A fibroblast culture was established from a normal human skin biopsy. Platelet gel was prepared from platelet-rich plasma (PRP) following the addition of calcified thrombin solution. Cell proliferation was measured by MTT assay.

Results: We showed that platelet gel, PRP, and thrombin stimulate the proliferation of dermal fibroblasts, and that stimulation by PRP is dose dependent.

Conclusion: Platelet gel can be used to treat chronic skin defects.

MeSH terms

  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Dermis / cytology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / physiology
  • Gels
  • Humans
  • Platelet-Rich Plasma*
  • Thrombin / pharmacology*


  • Gels
  • Thrombin